Synergic and antibiofilm effects of melampodium divaricatum l. (asteracae) essential oil upon bacteria associated with dental caries

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Raquel Regina Duarte Moreira
Caio Humberto Perego
José Ricardo Soares de Oliveira
Thaís da Silva Moraes
Carlos Henrique Gomes Martins

Resumo

Melampodium divaricatum is an herbaceous species, naturally occurring in the Northeast regions of Brazil and is known for its medicinal value and the action against oral pathogens. The objective of this study was to verify the synergistic effect and activity against biofilm formation of the EO from M. divaricatum against bacteria associated with dental caries. The checkerboard assays was used to investigate the in vitro antimicrobial efficacy of the combination of Chlorhexidine dichlorohydrate with M. divaricatum EO. The microtitration plate assay was used for determination the Minimum Inhibitory Concentration of Biofilm (MICB50) of essential oil against the bacteria. The synergistic effect and activity against biofilm formation of the essential oil (EO) from M. divaricatum against bacteria associated with dental caries were observed. The combination effects of EO with chlorhexidine were additive to L. casei, antagonic to S. mutans and indifferent to S. sobrinuns and S. mitis. The antibiofilm activity revealed significant results MICB50 values (200 at 400 µg/mL). This report suggests that the EO is a promising natural product to develop novel therapeutic strategies to fight against cariogenic bacteria.

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Como Citar
Moreira, R. R. D., Perego, C. H. ., Oliveira, J. R. S. de, Moraes, T. da S. ., & Martins, C. H. G. . (2022). Synergic and antibiofilm effects of melampodium divaricatum l. (asteracae) essential oil upon bacteria associated with dental caries. Revista Brasileira Multidisciplinar, 25(1), 121-128. https://doi.org/10.25061/2527-2675/ReBraM/2022.v25i1.753
Seção
Comunicação Breve
Biografia do Autor

Raquel Regina Duarte Moreira, Faculdade de Ciências Farmacêuticas, UNESP

Departamento de Princípios Ativos Naturais e Toxicologia. FARMACOGNOSIA

Caio Humberto Perego, Faculdade de Ciências Farmacêuticas, UNESP- Univ. Estadual Paulista

Departamento de Princípios Ativos Naturais e Toxicologia

Referências

AGRA MF, SILVA KN, BASÍLIO IJLD, FREITAS PF, BARBOSA-FILHO JM. Survey of medicinal plants used in the region Northeast of Brazil. Braz. J. Pharmacog. v. 18, p. 472-508, 2008.

AGUIAR GP, CARVALHO CE, MARTINS MHG, WAKABAYASHI KAL, GROPPO M, MARTINS CH, CUNHA WR, CROTTI AE. Antimicrobial activity of selected essential oils against cariogenic bacteria. Nat. Prod. Res. v. 27, p. 1668-72, 2013.

ALBERTSSON KW, PERSSON A, VAN DIJKEN JWV. “Effect of essential oils containing and alcohol-free chlorhexidine mouthrinses on cariogenic microorganisms in human saliva”. Acta Odontol. Scand. p. 883-91, 2013.

BERSAN SMF, GALVÃO LCC, GOES VFF, SARTORATTO A, FIGUEIRA GM, REHDER VLG, ALENCAR SM, DUARTE RMT, ROSALEN PL, DUARTE MCT. Action of essential oils from Brazilian native and exotic medicinal species on oral biofilms. BMC. v.14, p. 1-12, 2014.

BOTELHO MA, NOGUEIRA NAP, BASTOS GM, FONSECA SGC, LEMOS TLG, MATOS FJA, MONTENEGRO D, HEUKELBACH J, RAO VS, BRITO GAC. “Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens”. Braz. J. Med. Biol. Res. v. 40, p. 349-56, 2007.

CREVELIN EJ, CAIXETA SC, DIAS HJ, GROPPO M, CUNHA WR, MARTINS CHG, CROTTI AEC. Antimicrobial Activity of the Essential Oil of Plectranthus neochilus against Cariogenic Bacteria. Evid. Based Complement. Alternat. Med. p. 1-6, 2015.

CUNHA LCS, DE MORAIS AL, MARTINS CHG, MARTINS MM, CHANG R, AQUINO FJT, OLIVEIRA A, MORAES TS, MACHADO FC, SILVA CV, NASCIMENTO, E.A. Chemical composition, cytotoxic and antimicrobial activity of essential oils from Cassia bakeriana Craib. against aerobic and anaerobic oral pathogens. Molecules. v. 18, p. 4588-98, 2013.

FURIGA A, LONVAUD-FUNEL A, DORIGNAC G, BADET C. “In vitro anti-bacterial and anti-adherence effects of natural polyphenolic compounds on oral bacteria”. J. Applied Microbioly. v. 105, p. 1470-6, 2008.

Galvão, L.C.C., Furletti, V.F., Bersan, S.M.F., Cunha, M.G., Ruiz, A.L.T.G., Carvalho, J.E., Sartoratto, A., Rehder, V.L.G., Figueira, G.M., Duarte, M.C.T., Ikegaki, M., Alencar, S.M. & Rosalen, P.L. Antimicrobial Activity of Essential Oils against Streptococcus mutans and their Antiproliferative Effects. Evid. Based Complement. Alternat. Med. p. 1-12, 2012.

HENDRY ER, WORTHINGTON T, CONWAY BR, LAMBERT PA. Antimicrobial efficacy of eucalyptus oil and 1.8-cineole against microorganisms grow in planktonic and biofilm cultures. J. Antimicrob. Chemother. v. 64, p. 1219-25, 2009.

KOUIDHI B, ZMANTAR T, BAKHROUF A. “Anticariogenic and cytotoxic activity of clove essential oil (Eugenia caryophyllata) against a large number of oral pathogens”. Annals of Microbiology. v. 60, p. 599-604, 2010.

LEWIS RE, DIEKEMA DJ, MESSER SA, PFALLER MA, KLEPSER ME. Comparison of Etest, chequerboard dilution and time-kill studies for the detection of synergy or antagonism between antifungal agents tested against Candida species. J. Antimicrob. Chemother. v. 49, p. 345-51, 2002.

Lorenzi, H. “Plantas Daninhas do Brasil”: Terrestres, Aquáicas, Parasitas e Tóxicas , 3rd edn., Instituto Plantarum, Nova Odessa, SP, 2002.

MACIÀ MD, ROJO-MOLINERO E, OLIVER A. Antimicrobial susceptibility testing in biofilm-growing bacteria. Clin. Microbiol. Infect. v. 20, p. 981-90, 2014.

MAH TFC, O'TOOLE GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. v. 9, p. 34-9, 2001.

MOREIRA RRD, MARTINS GZ, BOTELHO VT, SANTOS LE CAVALEIRO C, SALGUEIRO L, ANDRADE G, MARTINS CHG. Composition and Activity against Oral Pathogens of the Essential Oil of Melampodium divaricatum (Rich.) DC. Chem. Biod. v. 11, p. 438-44, 2014.

PELISSARI GP, PIETRO RCLR, MOREIRA RRD. Atividade antibacteriana do óleo essencial de Melampodium divaricatum (Rich.) DC., Asteraceae. Braz. J. Pharmacog. v. 20, p. 70-4, 2010.

POLONIO RE, MERMEL LA, PAQUETTE GE, SPERRY JF. Eradication of biofilm-forming Staphylococcus epidermidis (RP62A) by a combination of sodium salicylate and vancomycin. Antimicrob. Agents Chemother. v. 45, p. 3262-6, 2001.

STEWART OS & COSTERTON JW. Antibiotic resistance of bacteria in biofilms. The Lancet. v. 11, p. 559-62, 2001.

WEI G-X, CAMPAGNA AN & BOBEK LA. Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm. J. Antimicrob. Chemother. v. 57, p. 1100-9, 2006.