O uso de fluido oral para detecção de drogas com interesse forense
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As análises toxicológicas forenses são valiosas ferramentas para o rastreamento e confirmação de substâncias de interesse forense em diferentes matrizes biológicas. As drogas de abuso representam um problema de saúde pública mundial, que podem resultar em acidentes e mortes. Convencionalmente, sangue e urina são as principais matrizes biológicas para realização das análises toxicológicas. O fluido oral (OF) é, hoje em dia, uma das matrizes biológicas alternativas mais comumente utilizadas para testes clínicos e forenses de drogas. Uma das características interessantes do OF é que a droga original é, frequentemente, a substância predominante detectada na amostra. Além da coleta ser simples e não invasiva. Diferentes métodos analíticos para detecção de drogas em amostras biológicas alternativas estão disponíveis. Este artigo teve como objetivo resumir quais são as principais vantagens do uso de OF para detecção de drogas e mostrar os métodos analíticos mais empregados nos últimos 5 anos. Para isso, uma busca foi realizada nas bases de dados BVS, PubMed e Science Direct. O reconhecimento de estudos de OF como uma matriz biológica eficiente e segura para detecção de diversas drogas, torna esta matriz cada vez mais importante nesse cenário. No entanto, os testes utilizando esta matriz biológica ainda são bem menores em comparação com o sangue e a urina.
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AMARATUNGA, P.; LORENZ LEMBERG, B.; LEMBERG, D. Quantitative Measurement of Synthetic Cathinones in Oral Fluid. Journal of Analytical Toxicology, v. 37, n. 9, p. 622–628, 1 nov. 2013.
ANVISA. Novas Substâncias Psicoativas (NSP). Disponível em: <https://www.gov.br/anvisa/pt-br/assuntos/medicamentos/controlados/novas-substancias/novas-substancias>. Acesso em: 19 nov. 2022.
ARES, A. M. et al. A fast bioanalytical method based on microextraction by packed sorbent and UPLC-MS/MS for determining new psychoactive substances in oral fluid. Talanta, v. 174, p. 454–461, 1 nov. 2017.
ARES-FUENTES, A. M. et al. Determination of synthetic opioids in oral fluid samples using fabric phase sorptive extraction and gas chromatography-mass spectrometry. Journal of Chromatography. A, v. 1663, p. 462768, 25 jan. 2022.
AVCIOGLU, G. et al. Evaluation of the diagnostic performance of an oral fluid screening test device for substance abuse at traffic controls. Clinical Biochemistry, v. 93, p. 112–118, 1 jul. 2021.
AXELSSON, M. A. B. et al. Retrospective identification of new psychoactive substances in patient samples submitted for clinical drug analysis. Basic & Clinical Pharmacology & Toxicology, v. 131, n. 5, p. 420–434, nov. 2022.
BAHMANABADI, L. et al. Quantitative determination of methamphetamine in oral fluid by liquid-liquid extraction and gas chromatography/mass spectrometry. Hum Exp Toxicol, p. 195–202, 2017.
BASSOTTI, E. et al. A new LC-MS/MS confirmation method for the determination of 17 drugs of abuse in oral fluid and its application to real samples. Forensic Science International, v. 312, p. 110330, jul. 2020.
BUENO, L. H. P. et al. Oral fluid as an alternative matrix to determine ethanol for forensic purposes. Forensic Science International, v. 242, p. 117–122, 1 set. 2014.
BUSARDÒ, F. P.; JONES, A. W. Interpreting γ-hydroxybutyrate concentrations for clinical and forensic purposes. Clinical Toxicology (Philadelphia, Pa.), v. 57, n. 3, p. 149–163, mar. 2019.
CAO, J. et al. Pharmacokinetics of meperidine (pethidine) in rabbit oral fluid: correlation with plasma concentrations after controlled administration. Die Pharmazie, v. 73, n. 6, p. 324–328, 1 jun. 2018.
CHEN, L. et al. Pharmacokinetics of selegiline, R-methamphetamine, R-amphetamine, and desmethylselegiline in oral fluid after a single oral administration of selegiline. Drug Testing and Analysis, v. 11, n. 6, p. 898–905, jun. 2019.
CHIAPPIN, S. et al. Saliva specimen: A new laboratory tool for diagnostic and basic investigation. Clinica Chimica Acta, v. 383, n. 1, p. 30–40, 1 ago. 2007.
CHINAGLIA, K. DE O. et al. Development of analytical method for the determination of methylphenidate, the analog ethylphenidate and their metabolite ritalinic acid in oral fluid samples by micro-QuEChERS and liquid chromatography-tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, v. 1205, p. 123330, 1 ago. 2022.
CONCHEIRO, M. et al. Determination of MDMA, MDA, MDEA and MBDB in oral fluid using high performance liquid chromatography with native fluorescence detection. Forensic Science International, Detection of Drugs in Oral Fluid. v. 150, n. 2, p. 221–226, 10 jun. 2005.
CONCHEIRO, M.; SHAKLEYA, D. M.; HUESTIS, M. A. Simultaneous analysis of buprenorphine, methadone, cocaine, opiates and nicotine metabolites in sweat by liquid chromatography tandem mass spectrometry. Analytical and bioanalytical chemistry, v. 400, n. 1, p. 69–78, abr. 2011.
DAMS, R. et al. Oral fluid as an alternative matrix to monitor opiate and cocaine use in substance-abuse treatment patients. Drug and Alcohol Dependence, v. 87, n. 2, p. 258–267, 16 mar. 2007.
DESHARNAIS, B. et al. A threshold LC-MS/MS method for 92 analytes in oral fluid collected with the Quantisal® device. Forensic Science International, v. 317, p. 110506, dez. 2020.
FENG, X. et al. LC-MS-MS with Post-Column Reagent Addition for the Determination of Zolpidem and its Metabolite Zolpidem Phenyl-4-carboxylic Acid in Oral Fluid after a Single Dose. Journal of Analytical Toxicology, v. 41, n. 9, p. 735–743, 1 nov. 2017.
FENG, X. et al. Zolpidem and zolpidem phenyl-4-carboxylic acid pharmacokinetics in oral fluid after a single dose. Drug Testing and Analysis, v. 11, n. 7, p. 1076–1082, jul. 2019.
FERNÁNDEZ, P. et al. Simultaneous determination of 20 drugs of abuse in oral fluid using ultrasound-assisted dispersive liquid-liquid microextraction. Analytical and Bioanalytical Chemistry, v. 411, n. 1, p. 193–203, jan. 2019.
GERACE, E. et al. Determination of cannabinoids in urine, oral fluid and hair samples after repeated intake of CBD-rich cannabis by smoking. Forensic Science International, v. 318, p. 110561, 1 jan. 2021.
GJERDE, H. et al. Detection of illicit drugs in oral fluid from drivers as biomarker for drugs in blood. Forensic Science International, v. 256, p. 42–45, nov. 2015.
GORZIZA, R. et al. Extraction of dried oral fluid spots (DOFS) for the identification of drugs of abuse using liquid chromatography tandem mass spectrometry (LC-MS/MS). Forensic Chemistry, v. 19, p. 100254, 1 jun. 2020.
GORZIZA, R. P. et al. A systematic review of quantitative analysis of cannabinoids in oral fluid. Journal of Forensic Sciences, v. 66, n. 6, p. 2104–2112, 2021.
GRUMANN, C. et al. Method validation and preliminary pharmacokinetic studies on the new designer stimulant 3-fluorophenmetrazine (3-FPM). Drug Testing and Analysis, v. 11, n. 7, p. 1009–1017, jul. 2019.
JACQUES, A. L. B.; SANTOS, M. K. D.; LIMBERGER, R. P. Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse. Journal of Forensic Sciences, v. 64, n. 6, p. 1906–1912, nov. 2019.
KAHL, J. M. M. et al. Quantification of amphetamine and derivatives in oral fluid by dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, v. 196, p. 113928, 20 mar. 2021.
KROTULSKI, A. J. et al. Dibutylone (bk-DMBDB): Intoxications, Quantitative Confirmations and Metabolism in Authentic Biological Specimens. Journal of Analytical Toxicology, v. 42, n. 7, p. 437–445, 1 set. 2018a.
KROTULSKI, A. J. et al. N-Ethyl Pentylone (Ephylone) Intoxications: Quantitative Confirmation and Metabolite Identification in Authentic Human Biological Specimens. Journal of Analytical Toxicology, v. 42, n. 7, p. 467–475, 1 set. 2018b.
KROTULSKI, A. J. et al. The Detection of Novel Stimulants in Oral Fluid from Users Reporting Ecstasy, Molly and MDMA Ingestion. Journal of Analytical Toxicology, v. 42, n. 8, p. 544–553, 1 out. 2018c.
LA MAIDA, N. et al. Determination of the Synthetic Cannabinoids JWH-122, JWH-210, UR-144 in Oral Fluid of Consumers by GC-MS and Quantification of Parent Compounds and Metabolites by UHPLC-MS/MS. International Journal of Molecular Sciences, v. 21, n. 24, p. E9414, 10 dez. 2020.
LANGEL, K. et al. Comparison of drug concentrations between whole blood and oral fluid. Drug Testing and Analysis, v. 6, n. 5, p. 461–471, 2014.
LIN, L. et al. Quantitation of Δ8-THC, Δ9-THC, Cannabidiol and 10 Other Cannabinoids and Metabolites in Oral Fluid by HPLC-MS-MS. Journal of Analytical Toxicology, v. 46, n. 1, p. 76–88, 14 fev. 2022.
LIUT, J. et al. Evaluation of RapidSTAT®, DrugWipe® 6S, DrugScreen® 5TK and DrugScreen® 7TR for on-site drug testing in German police roadside traffic patrol. Drug Testing and Analysis, v. 14, n. 8, p. 1407–1416, ago. 2022.
LOOD, Y. et al. Relationship between testosterone in serum, saliva and urine during treatment with intramuscular testosterone undecanoate in gender dysphoria and male hypogonadism. Andrology, p. 86–93, 2018.
MARCHEI, E. et al. Stability and Degradation Pathways of Different Psychoactive Drugs in Neat and in Buffered Oral Fluid. Journal of Analytical Toxicology, v. 44, n. 6, p. 570–579, 31 jul. 2020.
MILLER, B.; KIM, J.; CONCHEIRO, M. Stability of synthetic cathinones in oral fluid samples. Forensic Science International, Special Issue on the 54th Annual Meeting of the International Association of Forensic Toxicologists (TIAFT) Brisbane from August 28 to September 1, 2016. v. 274, p. 13–21, 1 maio 2017.
MORTIER, K. A. et al. Simultaneous, quantitative determination of opiates, amphetamines, cocaine and benzoylecgonine in oral fluid by liquid chromatography quadrupole-time-of-flight mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, v. 779, n. 2, p. 321–330, 5 nov. 2002.
MOZANER BORDIN, D. et al. Técnicas de preparo de amostras biológicas com interesse forense. Scientia Chromatographica, v. 7, p. 125–143, 1 jan. 2015.
NICOLAOU, A. G. et al. Analysis of cannabinoids in conventional and alternative biological matrices by liquid chromatography: Applications and challenges. Journal of Chromatography. A, v. 1651, p. 462277, 16 ago. 2021.
PACIFICI, R. et al. Determination of cannabinoids in oral fluid and urine of “light cannabis” consumers: a pilot study. Clinical Chemistry and Laboratory Medicine, v. 57, n. 2, p. 238–243, 19 dez. 2018.
PALMER, K. L.; KRASOWSKI, M. D. Alternate Matrices: Meconium, Cord Tissue, Hair, and Oral Fluid. Methods in Molecular Biology (Clifton, N.J.), v. 1872, p. 191–197, 2019.
PALMQUIST, K. B.; SWORTWOOD, M. J. Data-independent screening method for 14 fentanyl analogs in whole blood and oral fluid using LC-QTOF-MS. Forensic Science International, v. 297, p. 189–197, abr. 2019.
PALMQUIST, K. B.; SWORTWOOD, M. J. Quantification of fentanyl analogs in oral fluid using LC-QTOF-MS. Journal of Forensic Sciences, v. 66, n. 5, p. 1871–1878, 2021.
PASCALI, J. P. et al. Oral fluid analysis to monitor recent exposure to synthetic cannabinoids in a high-risk subpopulation. Journal of Forensic Sciences, v. 67, n. 5, p. 1932–1937, set. 2022.
PUJADAS, M. et al. A simple and reliable procedure for the determination of psychoactive drugs in oral fluid by gas chromatography–mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, Hyphenated Techniques in Pharmaceutical and Biomedical Analysis 2006. v. 44, n. 2, p. 594–601, 28 jun. 2007.
REISINGER, A. J. et al. Oral Cavity Fluid as an Investigative Approach for Qualitative and Quantitative Evaluations of Drugs in Postmortem Subjects. J Anal Toxicol, p. 444–451, 2019.
RISOLUTI, R. et al. Miniaturized analytical platform for cocaine detection in oral fluids by MicroNIR/Chemometrics. Talanta, v. 202, p. 546–553, 1 set. 2019.
RUBIO, N. C. et al. Perfil de alcaloides de la hoja de coca en el fluido oral de un mascador de hoja de coca y un bebedor de té de coca: Estudio preliminar. Acta toxicol. argent, p. 72–80, 2019a.
RUBIO, N. C. et al. Development of a Reliable Method for Assessing Coca Alkaloids in Oral Fluid by HPLC-MS-MS. Journal of Analytical Toxicology, v. 43, n. 3, p. 196–202, 1 abr. 2019b.
SCHERER, J. N. et al. Reliability of point-of-collection testing devices for drugs of abuse in oral fluid: A systematic review and meta-analysis. Journal of Pharmaceutical and Biomedical Analysis, v. 143, p. 77–85, 5 set. 2017.
SMITH, C. R.; SWORTWOOD, M. J. Analysis of methylphenidate, ethylphenidate, lisdexamfetamine, and amphetamine in oral fluid by liquid chromatography-tandem mass spectrometry. Journal of Forensic Sciences, v. 67, n. 2, p. 669–675, mar. 2022.
SOARES, S. et al. New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling. Pharmaceuticals (Basel, Switzerland), v. 14, n. 12, p. 1284, 8 dez. 2021.
TRUVER, M. T.; PALMQUIST, K. B.; SWORTWOOD, M. J. Oral Fluid and Drug Impairment: Pairing Toxicology with Drug Recognition Expert Observations. Journal of Analytical Toxicology, v. 43, n. 8, p. 637–643, 10 set. 2019.
UNODC, U. N. O. ON D. AND C. World drug report 2013 :New Psychoactive Substances: Concepts and Definitions. Vienna, Austria: [s.n.].
UNODC, U. N. O. ON D. AND C. World drug report 2017: Global Overview of Drug Demand and Supply. Vienna, Austria: [s.n.].
VINCENTI, F. et al. Simultaneous Quantification of 25 Fentanyl Derivatives and Metabolites in Oral Fluid by Means of Microextraction on Packed Sorbent and LC-HRMS/MS Analysis. Molecules (Basel, Switzerland), v. 26, n. 19, p. 5870, 28 set. 2021.
YONAMINE, M. et al. Solid-phase micro-extraction–gas chromatography–mass spectrometry and headspace-gas chromatography of tetrahydrocannabinol, amphetamine, methamphetamine, cocaine and ethanol in saliva samples. Journal of Chromatography B, 40th Annual International Meeting of The International Association of Forensic Toxicologists. v. 789, n. 1, p. 73–78, 5 jun. 2003.