EFFICACY AND SAFETY OF INNOVATIVE SUNSCREEN (E) -4- (TERT-BUTYL) -N '- ((E) -3- (4-METHOXYPHENYL) ALLYLIDENE) BENZIDRAZIDA (TBMAB)
Barra lateral del artículo
Contenido principal del artículo
Resumen
The constant or repeated unprotected exposure to solar radiation can result in the onset of various harmful effects, such as inflammation, genetic mutation and hyperpigmentation, even than previous photoaging. The use of photoprotective formulations is one of the most effective tools to avoid this. However, most of the UV filters are unstable to UV radiation, can even permeate the skin and cause hepatotoxic effects. The aim of this study was to assess the efficacy and safety of a innovative sunscreen substance (E) -4- (tert-butyl) -N '- ((E) -3- (4-methoxyphenyl) allylidene) benzhydrazide. Methodology: The sunscreen formulation containing the innovative substance was subjected to various stress conditions: 45±2° C, 5±2° C and exposure to sunlight. These samples were evaluated in relation to its rheology, pH, viscosity, density and SPF to determine its stability. The assessment of TBMAB release from the formulation was performed in Microette equipment. Hepatoma cells (HepG2) were used to determine the TBMAB cytotoxicity. Results and Discussion: In the stability study, no significant changes were observed in the formulation. The maximum concentration of TBMAB released from the formulation was 3.63 µg.cm-2. Also, it was evident the cytotoxic effect on the concentrations well above 3.63 µg.cm-2,displaying the safety of this substance, considering HepG2 cells. Conclusion: These results demonstrate that TBMAB is a suitable innovative substance to be used in suscreens, exhibiting stability, desired SPF value and safety, considering HepG2 cells. Thus, it could be a new alternative to prevent skin cancer.
Descargas
Detalles del artículo
Esta obra está bajo una licencia internacional Creative Commons Atribución-SinDerivadas 4.0.
- O(s) autor(es) autoriza(m) a publicação do artigo na revista;
• O(s) autor(es) garante(m) que a contribuição é original e inédita e que não está em processo de avaliação em outra(s) revista(s);
• A revista não se responsabiliza pelas opiniões, ideias e conceitos emitidos nos textos, por serem de inteira responsabilidade de seu(s) autor(es);
• É reservado aos editores o direito de proceder ajustes textuais e de adequação do artigo às normas da publicação.
Os conteúdos da Revista Brasileira Multidisciplinar – ReBraM estão licenciados sob uma Licença Creative Commons 4.0 by.
Qualquer usuário tem direito de:
- Compartilhar — copiar, baixar, imprimir ou redistribuir o material em qualquer suporte ou formato.
- Adaptar — remixar, transformar, e criar a partir do material para qualquer fim, mesmo que comercial.
De acordo com os seguintes termos:
- Atribuição — Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de maneira alguma que sugira ao licenciante a apoiar você ou o seu uso.
- Sem restrições adicionais — Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.
Autores concedem à ReBraM os direitos autorais, com o trabalho simultaneamente licenciado sob a Licença Creative Commons 4.0 by. , que permite o compartilhamento do trabalho com reconhecimento da autoria e publicação inicial nesta revista.
Citas
Rass, K., Reichrath, J. UV damage and DNA repair in malignant melanoma and nonmelanoma skin cancer. Advances in Experimental Medicine and Biology. 624, 162–78 (2008).
Reis Junior, M.A., Reis, J.D.S., Oliveira, J.A.D., Corrêa, M.A. molecular targets of new drugs for prevention skin. World J Pharm Pharm Sci. 5(7), 1574–85, (2016).
Siegel, R., Miller, K., Jemal, A. Cancer statistics , 2015 . CA Cancer J Clin 65(1), 29 (2015).
Armstrong, B.K., Kricker, A. The epidemiology of UV induced skin cancer. J Photochem Photobiol B Biol. 63(1), 8–18 (2001).
Berneburg, M., Plettenberg, H., Krutmann, J. Photoaging of human skin. Photodermatol Photoimmunol Photomed. 16(6), 239–44 (2000).
Boukamp, P. Skin Cancer (Non-Melanoma). Hum Cell Cult. 1, 251–257 (1999).
Gruijl, F.R. Skin cancer and solar UV radiation. Eur J Cancer. 35(14), 2003–9 (1999).
Dennis, L.K., Freeman, L.E.B., VanBeek, M.J. Sunscreen Use and the Risk for Melanoma: A Quantitative Review. A of Internal Medicine. 139, 966–978, (2003).
Gordon, R. Skin cancer: An overview of epidemiology and risk factors. Semin Oncol Nurs. 29(3), 160–9 (2013).
Soehnge, H., Ouhtit, A., Ananthaswamy, O.N. Mechanisms of induction of skin cancer by UV radiation. Front Biosci 2(1), 538-51 (1997).
Wright, T.I., Spencer, J.M., Flowers, F.P. Chemoprevention of nonmelanoma skin cancer. Journal of the American Academy of Dermatology. 54, 933–46 (2006).
Reis, J.S., Corrêa, M.A., Chung, M.C., Dos Santos, J.L. Synthesis, antioxidant and photoprotection activities of hybrid derivatives useful to prevent skin cancer. Bioorganic Med Chem. Elsevier Ltd 22(9), 2733–8 (2014).
Isaac, V.L.B., Cefali, L.C., Chiari, B.G., Oliveira, C.C.L.G., Salgado, H.R.N., Corrêa, M.A. Protocolo para ensaios físico-químicos de estabilidade de fitocosméticos. Rev Ciencias Farm Basica e Apl. 29(1), 81–96 (2008).
Dutra, E.A., Almança, D., Kedor, E.R.M., Inês, M., Miritello, R. Determination of sun protection factor ( SPF ) of sunscreens by ultraviolet spectrophotometry. Brazilian J Pharm Sci. 40(3), 381–5 (2004).
Gallegos, C., Franco, J.M. Rheology of food, cosmetics and pharmaceuticals. Vol. 4, Current Opinion in Colloid and Interface Science. 288–93 (1999).
Franz, T.J. Percutaneous Absorption. On the Relevance of in Vitro Data. J Invest Dermatol 64(3), 190–5 (1975).
Lehman, P.A., Raney, S.G., Franz, T.J. Percutaneous absorption in man: In vitro-in vivo correlation. Skin Pharmacol Physiol. 24(4), 224–30 (2011).
ANVISA. Guía para validação de métodos analíticos e bioanalíticos. Resolução RE n 899, 29 maio 2003 Available from <http://redsang.ial.sp.gov.br/site/docs_leis/vm/vm1.pdf>
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 65(1), 55–63 (1983).
Abe, K., Matsuki, N. Measurement of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction activity and lactate dehydrogenase release using MTT. Neurosci Res. 38(4), 325–9 (2000).
Sthle, L., Wold, S. Analysis of variance (ANOVA). Chemometrics and Intelligent Laboratory Systems. 6, 259–72 (1989).
Springsteen, A., Yurek, R., Frazier, M., Carr, K.F. In vitro measurement of sun protection factor of sunscreens by diffuse transmittance. Analytica Chimica Acta. 155–64, (1999).
ANVISA. Guia para a Realização de Estudos de Estabilidade. Available from: <http://200.189.113.52/ftp/Visa/GuiaRealizEstudos.pdf>
Corrêa, M.A. Cosmetologia: ciência e técnica. Livraria e Editora Medfarma. 282–4, (2012).
Alves, M.M. A química e a reologia no processamento dos alimentos. Ciência e Técnica 37–61, (2004).
Barry, B.W., Warburton, B. Some rheological aspects of cosmetics. J Soc Cosmet Chem 19(11), 725–44 (1968).
Shehata AB, Rizk MS, Rend EA. Certification of caffeine reference material purity by ultraviolet/visible spectrophotometry and high-performance liquid chromatography with diode-array detection as two independent analytical methods. J Food Drug Anal. 2016 Oct;24(4):703-715.
Leone, Beatriz Alves. "Determinação da seletividade no sinergismo entre filtros solares sintéticos e óleo de café verde." Tese (mestrado em Ciências Farmacêuticas) – Faculdade de Ciências Farmacêuticas, Universidade Júlio de Mesquita Filho, UNESP, Araraquara. (2018).